Table 1.

The frequencies of splenic and peritoneal CD19⁺IL-10⁺ cells within total CD19⁺ B cells were significantly reduced in AP females on gd10.

CD19+IL-10+ within CD19+	Spleen					Peritoneum
	gd0	gd2	gd5	gd10	gd14	gd0	gd2	gd5	gd10	gd14
Virgin	11.0					19.7
NP	14.5	10.5	12.7	15.4	13.1	20.2	24.7	27.3	24.4	19.0
AP	10.1	13.1	17.2	9.7*	13.2	25.0	23.1	24.5#	11.7**	15.5

The frequencies of CD19⁺IL-10⁺ B cells within total CD19⁺ B cells were determined in spleen and peritoneum from virgin (n = 5 per organ), NP (n = 4–7 per organ), and AP (n = 5–6 per organ) females at different gestation days (gds). For flow cytometry analysis, a first gate was set on all mononuclear cells, followed by a representation of CD19⁺ cells within this gate. Next, a second gate was set on all CD19⁺ cells, and within the second gate IL-10 was plotted against CD19. Data are displayed as medians.

Statistical analysis was performed using the Mann–Whitney-U test (*p ≤ 0.05 vs NP; **p ≤ 0.01 vs NP; and ^#p ≤ 0.05 vs virgin).

NP, normal pregnant; AP, abortion prone.