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Differentiation of hESCs into insulin-producing cells. Phase contrast microscopy of undifferentiated hESC colonies (A), Embryoid bodies; EBs (B) and differentiated cells at D21 (C), D28 (E) and D46 (E,F), arrows indicate small islet-like cell clusters. Scale bars, 300 μm (A); 100 μm (B–F).
