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. 2018 May 15;6:49. doi: 10.3389/fcell.2018.00049

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Copyright © 2018 Rattananinsruang, Dechsukhum and Leeanansaksiri.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescence microscopic imaging of immunostaining for nestin, somatostatin, glucagon and C-peptide. The cells after 46 days of differentiation were stained with antibodies against nestin (red), somatostatin (red), glucagon (green) and C-peptide (red), followed by appropriate secondary antibodies conjugated to fluorochromes (blue, DAPI for nuclear staining; red, Cy-3; green, FITC). A merge of both images shows localization of nestin (A), somatostatin (B), glucagon (B,C), and C-peptide (C). The images were taken at magnifications of ×20 (A,B) and ×10 (C).