Table 1.
Primer sequences and PCR conditions used in the real-time PCR.
| Gene | Primer Sequence (5'−3') | Annealing Temp. (°C) | Size (bp) | References |
|---|---|---|---|---|
| GAPDH | F: AGCCACATCGCTCAGACACC R: GTACTCAGCGGCCAGCATCG |
60 | 302 | Yao et al., 2006 |
| Nestin | F: CAGCTGGCGCACCTCAAGATG R: AGGGAAGTTGGGCTCAGGACTGG |
55 | 208 | Shim et al., 2007 |
| Pdx1 | F: CCCATGGATGAAGTCTACC R: GTCCTCCTCCTTTTTCCAC |
58 | 206 | Seeberger et al., 2006 |
| Hnf3β | F: CCACCACCAACCCCACAAAATG R: TGCAACACCGTCTCCCCAAAGT |
60 | 294 | Baharvand et al., 2006 |
| Ngn3 | F: GGTAGAAAGGATGACGCCTC R: CCGAGTTGAGGTCGTGCAT |
58 | 313 | Seeberger et al., 2006 |
| NeuroD1 | F: GCCCCAGGGTTATGAGACTATCACT R: CCGACAGAGCCCAGATGTAGTTCTT |
61 | 523 | Khoo et al., 2005 |
| Nkx6.1 | F: GTTCCTCCTCCTCCTCTTCCTC R: AAGATCTGCTGTCCGGAAAAAG |
53 | 381 | Segev et al., 2004 |
| GLUT2 | F: AGGACTTCTGTGGACCTTATGTG R: GTTCATGTCAAAAAGCAGGG |
55 | 231 | Segev et al., 2004 |
| Insulin | F: CTACCTAGTGTGCGGGGAAC R: CACAATGCCACGCTTCTG |
60 | ~150 | Yu et al., 2007 |