Table 3.
Multiple reaction monitoring parameters of peptides (and internal standard) selected for targeted analysis of hepatobiliary transporters.
| Transporter | Signature peptides | Molecular weight | Parent ion (z = 2) | Product ion (z = 1) | Fragmentor | Collision energy (V) |
|---|---|---|---|---|---|---|
| Mrp2 | LTIIPQDPILFSGSLR | 1770.08 | 885.7 | 1329.9 | 200 | 25 |
| Oct1 | GVALPETIEEAENLGR | 1697.84 | 849.7 | 1357.8 | 180 | 29 |
| Ntcp | AAATEDATPAALEK | 1358.42 | 680.0 | 915.5 | 140 | 18 |
| AAATEDATPAALEK∗ | 1366.42 | 684.0 | 923.0 | 140 | 23 | |
| Oatp1a4 | TFQFPGDIESSK | 1355.45 | 678.6 | 832.3 | 160 | 19 |
| Mate1 | LLLSGFQEELR | 1304.48 | 652.9 | 965.0 | 140 | 24 |
| Bsep | STALQLIQR | 1029.19 | 515.5 | 529.5 | 130 | 17 |
| Mdr1 | NTTGALTTR | 934 | 467.9 | 719.4 | 110 | 14 |
| Oatp1a1 | EENLGITK | 903 | 452.3 | 468.1 | 120 | 8 |
| Oatp1a2 | SVQPELK | 799.91 | 400.8 | 486.3 | 110 | 9 |
| UGT1A1 | SVFDQDPFLLR | 1336.48 | 668.8 | 575.8 | 150 | 18 |
∗Labeled amino acid residue of the internal standard.