Figure 1.

Free DNA is released from canine and didelphine granulocytes after T. cruzi infection. (A) Free DNA was measured in plasma of heparin blood after 2 h incubation at 37°C, 5% CO₂ (1:2 diluted with PBS). Significant higher amount of free DNA was detectable in opossum plasma by unpaired, two-tailed Student's t-Test (n = 5, ^****P < 0.0001). (B,C) Isolated granulocytes were incubated for 3 h with PBS as negative control and the cholesterol-depleting cyclodextrine (CD) as positive ET inducer or T. cruzi with MOI 2 or 5. Afterwards free DNA in supernatants as an indicator for ETs was measured with Pico Green test. (A) No significant difference was detected in the analyzed dogs by Friedman test (n = 3). (B) No significant difference was detected in the analyzed dogs by Friedman test (n = 3). (C) In contrast a significant difference between negative control and T. cruzi MOI 5 was detected in the analyzed opossums by Friedman test (P = 0.0174) followed by Dunn's multiple comparisons test (P = 0.0342) (n = 3, ^*P < 0.05).