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Neutrophil elastase detection in canine extracellular traps (ETs) Immunofluorescence microscopy allowed detection of neutrophil elastase (NE) in ETs after 180 min treatment with cholesterol-depleting cyclodextrine (CD) or T. cruzi (MOI = 5) as indicated. The isotype control was negative for NE and ETs. (blue = DNA [Hoechst], green = DNA/histone-1- complexes [ETs], pink = neutrophil elastase [NE]). Representative pictures are shown.
