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. 2018 May 15;9:974. doi: 10.3389/fmicb.2018.00974

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Copyright © 2018 Yu, Wang, Wang, Feng, Wu, Liu, Xin, Xiong, Liu and Shao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of M. hyopneumoniae EF-Tu surface display using flow cytometry. (A) Blank control, bacteria treated with PBS alone; negative control, bacteria treated with preimmune serum; Mhp strain 168, NJ, and WX: bacteria treated with anti-rEF-Tu serum. (B) The level of mean fluorescence intensity (MFI) of M. hyopneumoniae incubated with anti-rEF-Tu sera is expressed as the percentage of that incubated with preimmune sera. The asterisk above the charts stands for statistically significant differences.