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. 2018 May 15;9:984. doi: 10.3389/fmicb.2018.00984

Figure 1.

Figure 1

Time-dependent enhancement of cell-to-cell contact-mediated EBV transmission into epithelial cells. (A) Analysis of cell-to-cell contact-mediated EBV transmission into epithelial cells with a confocal laser scanning microscope. Akata− EBV-eGFP cells were cocultured with AGS (top) or NU-GC-3 cells (bottom) for various times. eGFP-positive, infected epithelial cells (white) were visualized by a confocal laser scanning microscope. The nuclei were counterstained with Hoechst 33342 (blue). Representative images are shown. Scale bars: 40 μm. (B) Summary of cell-to-cell contact-mediated EBV transmission. Akata− EBV-eGFP cells were cocultured with AGS (gray) or NU-GC-3 cells (black) for various times. The percentages of eGFP-positive, infected epithelial cells were analyzed by means of flow cytometry. The experiment was performed three times independently and the average values and their SD are shown for each condition.