Skip to main content
. 2018 May 15;9:984. doi: 10.3389/fmicb.2018.00984

Figure 3.

Figure 3

Effect of the blocking antibody for TGF-β on cell-to-cell contact-mediated EBV transmission. (A,B) Effect of the blocking antibody for TGF-β on cell-to-cell contact-mediated EBV transmission. Akata− EBV-eGFP cells were cocultured with AGS cells (A) or NU-GC-3 cells (B) for 5 days in the presence of the blocking antibody for human TGF-β (0.5, 1.0, 2.0, 5.0, 10.0 μg/ml). As a control, an antibody for ER was used. The percentages of eGFP-positive, infected epithelial cells were analyzed by means of flow cytometry. The experiment was performed three times independently and the average and its SD are shown in each condition. *P < 0.05; **P < 0.01 vs. respective control (Student's t-test). (C) Effect of the blocking antibody for TGF-β on induction of lytic cycle in cocultured B cells. Akata− EBV-eGFP cells were cocultured with AGS (gray) or NU-GC-3 cells (black) for 5 days in the presence of 10.0 μg/ml anti-human TGF-β. As a control, an anti-estrogen receptor (ER) antibody was used. B cells were harvested, and percentage of gp350-positive cells was analyzed by immunofluorescence staining. The experiment was performed three times independently and the average values and their SD are shown for each condition. **P < 0.01 vs. respective control (Student's t-test).