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. 2018 May 15;9:497. doi: 10.3389/fphar.2018.00497

FIGURE 1.

FIGURE 1

Effects of acacetin on cell viability and apoptosis in neonatal rat cardiomyocytes and H9C2 cardiomyoblasts subjected to hypoxia/reoxygenation. (A) Flow cytometry graphs showing cell viability and apoptosis populations in neonatal rat cardiomyocytes without (control) or with hypoxia/reoxygenation (H/R) exposure in the absence or presence of 3 μM acacetin. Cells were treated with FITC-labeled Annexin V and PI staining [viability (V); dead cells (D); late apoptosis (LA); early apoptosis (EA)]. (B) Mean percent values of cell viability, early apoptosis, late apoptosis, and dead cells in neonatal rat cardiomyocytes without or with hypoxia/reoxygenation (H/R) in the absence or presence of 0.3, 1, or 3 μM acacetin. (C) Flow cytometry graphs of H9C2 cardiomyoblasts with the treatment used in (A). (D) Mean percent values of cell viability, early apoptosis, late apoptosis, and dead cells in H9C2 cardiomyoblasts with the treatment used in (A). Data were expressed as mean ± SEM and analyzed by one-way ANOVA followed by Bonferroni-test (n = 5 individual experiments, ∗∗P < 0.01 vs. control; #P < 0.05, ##P < 0.01 vs. H/R alone).