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. 2018 May 15;9:497. doi: 10.3389/fphar.2018.00497

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Copyright © 2018 Wu, Li, Cui, Wu, Hong, Li, Wu, Jie, Wang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of acacetin on antioxidant-related proteins in cells with hypoxia/reoxygenation (H/R) exposure. Western blots and mean relative levels of Nrf2 (A), HO-1 (B), SOD1 (C), SOD2 (D) in neonatal rat cardiomyocytes without (control) or with hypoxia/reoxygenation exposure in the absence (V, vehicle) or presence of 0.3, 1, or 3 μM acacetin. Western blots and mean relative levels of Nrf2 (E), HO-1 (F), SOD1 (G), SOD2 (H) in H9C2 cardiomyoblasts without (control) or with hypoxia/reoxygenation exposure in the absence or presence of 0.3, 1, or 3 μM acacetin. Data were expressed as mean ± SEM and analyzed by one-way ANOVA followed by Bonferroni-test (n = 5 individual experiments, ^∗P < 0.05, ^∗∗P < 0.01 vs. control; ^#P < 0.05 vs. hypoxia/reoxygenation alone).