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. 2018 May 15;9:497. doi: 10.3389/fphar.2018.00497

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Copyright © 2018 Wu, Li, Cui, Wu, Hong, Li, Wu, Jie, Wang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of silencing Nrf2 on apoptosis- and inflammation-related proteins in cells with hypoxia/reoxygenation insult. Western blots and relative levels of Bcl-2 (A), Bax (B), and cleaved caspase-1 (C) in H9C2 cardiomyoblasts transfected with control siRNA or Nrf2 siRNA and subjected to hypoxia/reoxygenation insult in the absence (V, vehicle) or presence of 3 μM acacetin (Aca). Western blots and relative levels of IL-6 (D), TRL-4 (E), and IL-10 (F) in H9C2 cardiomyoblasts with the treatment used in (A–C). Data were expressed as mean ± SEM and analyzed by one-way ANOVA followed by Bonferroni-test (n = 5 individual experiments, ^∗P < 0.05, ^∗∗P < 0.01 vs. vehicle of control siRNA; ^##P < 0.01 vs. control siRNA with acacetin).