Table 3.
Quantification of vitamin B12 levels by HPLC-MS.
| ng Vitamin B12/mg protein | ||||
|---|---|---|---|---|
| Aerobic exponential | Aerobic stationary | Anaerobic | Biofilm | |
| PAO1 | 0nd | 0.32 ± 0.05 | 0nd | 3.72 ± 0.01 |
| PAET1 | 0nd | 0.67 ± 0.06 | 0nd | 1.72 ± 0.05 |
| PA14 | 0nd | 0.51 ± 0.02 | 0nd | 0.84 ± 0.01 |
Growth of P. aeruginosa PAO1, PAET1 and PA14 strains under aerobic (exponential OD550~0.5 and stationary OD550>2) and anaerobic conditions for 20 h (OD550~2) and under continuous-flow biofilm formation conditions (5 days). Values were normalized by protein concentration. Two independent experiments were performed, and the mean ± standard deviation is shown. nd, denotes Not-detected, below the technique detection limit.