Table 4.
Expression of nrdA, nrdJ, and nrdD genes of P. aeruginosa PAO1 and clinical isolates (PAET1 and PA14) strains with HU.
| Log-fold change (HU vs. H2O) | |||
|---|---|---|---|
| Class Ia nrdA | Class Ia nrdJ | Class III nrdD | |
| PAO1 | 9.19 ± 2.1 | 31.65 ± 3 | 50.46 ± 7.2 |
| PAET1 | 27.74 ± 2.1 | 7.63 ± 1.1 | 16.38 ± 0.5 |
| PA14 | 4.91 ± 0.8 | 1.18 ± 0.2 | 32.7 ± 0.9 |
Fold-change of nrd genes using qRT-PCR after 20 min of treatment with 30 mM HU compared with H2O. The gap gene was used as an internal standard. Two independent experiments were analyzed, and the standard deviation was annotated.