FIGURE 4.

Dasatinib induces changes in elasticity of HMVEC-L and HPAEC cells, as measured by AFM. Pseudo-colored reconstructed elasticity (each point showing the apparent Young’s modulus) maps of HMVEC-L (A,C) and HPAEC (B,D) cells prior to and 45 min after treatment with vehicle (0.1% DMSO) containing medium (med) as control or 100 nM dasatinib (das), respectively. Charts (E,F) represent average values of five parallel sets. Values were normalized to the pre-treatment state of each set. Cells were treated with dasatinib (das, 100 nM), imatinib (im, 5 μM; data not shown) or vehicle (0.1% DMSO) containing medium (ctrl) for both studied cell types.