FIGURE 5.

Rho-kinase inhibition partially reverses dasatinib-induced alterations in the barrier properties of HMVEC-L and HPAEC cells. Cells were treated with dasatinib (100 nM), dasatinib and Y27632 (10 μM), Y27632, dasatinib and fasudil (10 μM) or vehicle as control (ctrl: 0.1% DMSO) and CI was assessed (A,B). Permeability changes of HMVEC-L (C) and HPAEC (D) monolayers was determined after 24 h. Statistical analysis was performed using two-way ANOVA test (A,B) or one-way ANOVA (C,D) with Tukey post hoc multiple comparison; ^∗p < 0.05, ^‡p < 0.01, ⁺p < 0.001, ^∙p < 0.0001. Representative images of five (N = 5, A), four (N = 4, B) or three (N = 3, C,D) independent experiments are shown.