FIGURE 7.

Disruption of endothelial junctions in HPAEC cells is partially ameliorated by simultaneous ROCK inhibition. Cells were treated with dasatinib (100 nM), dasatinib and Y27632 (10 μM), Y27632 or vehicle (ctrl: 0.1% DMSO) for 2 h. Cells were fixed and probed with anti-VE-cadherin (red) or anti-ZO-1 antibodies (green), nuclei were counterstained with Hoechst 33342 (blue). (A) Representative images of three independent experiments are shown (N = 3). (B) Quantification of the immunofluorescence staining intensity (C) and staining continuity. Arrows indicate loss of junctional protein staining, while arrowheads indicate the gaps between cells. Data analysis was done by one-way ANOVA with Tukey post hoc test. Values are presented as mean ± SD; ^∗p < 0.05, ^‡p < 0.01, ^∙p < 0.0001 compared to ctrl (N = 4).