Table 1.
Statistics of sequencing, de novo assembly, and annotation of nuclear genomes.
Strain | I-30 | I-329 | I-566 |
Coverage by Illumina HiSeq-2500 | 264X | 234X | 219X |
Number of Illumina reads (after filtration) | 14,051,242 | 13,236,656 | 13,682,586 |
Average length of Illumina reads, nt | 218 | 205 | 184 |
Coverage by PacBio RSII | 59X | 60X | – |
Number of PacBio reads | 122,857 | 191,070 | – |
Average length of PacBio reads, nt | 5,596 | 3,655 | – |
Total contigs | 155 | 71 | 562 |
Number of contigs larger than 500 bp | 80 | 45 | 443 |
Contig N50, bp | 470,360 | 511,336 | 58,253 |
Largest contig, bp | 870,688 | 1,035,721 | 252,238 |
Total assembly length, bp | 11,587,783 | 11,587,876 | 11,502,012 |
Predicted protein-coding genes | 5,323 | 5,323 | 5,290 |
Predicted tRNA genes | 285 | 288 | 284 |
Ty elements | 472 | 474 | 594 |