Table 2.

PCR protocol of each gene.

Primer pair	Initial denaturation	Denaturation	Annealing	Extension	Cycles
HdC1/HdC2	94°C, 2 min	94°C, 30 s	52°C, 40 s	72°C, 30 s	35
TD2/TD5	95°C, 5 min	95°C, 45 s	52°C, 30 s	72°C, 1 min	31
ODF/ODR	95°C, 5 min	95°C, 45 s	52°C, 30 s	72°C, 1 min	31
AgmSq1/AgmSq2	94°C, 2 min	94°C, 30 s	52°C, 30 s	72°C, 1 min	30
AgD1/AgD2	94°C, 2 min	94°C, 30 s	52°C, 30 s	72°C, 1 min	30
Cad2F/Cad2R	94°C, 2 min	94°C, 30 s	52°C, 30 s	72°C, 1 min 30 s	30
BCf/BCr	95°C, 5 min	95°C, 30 s	58°C, 30 s	72°C, 1 min 30 s	31