Table 1.

Troubleshooting table.

Problem	Possible reason	Solution
Embryos are carried away during inserting.	Embryos are glued not enough.	Prepare thicker heptane glue to stable embryos.
	The tip is not sharp enough.	Adjust the tip size of micropipettes during preparation.
Body fluids flow out during injection.	Not enough Dehydration.	Prolong dehydration to 6-7 min.
	The flow from the micropipette tip is too high.	Use the micropipette with an appropriate tip size.
		Reduce the output pressure or the pulse duration.
Dextran-positive macropinosomes cannot be seen after 30 min of injection.	The micropipette tip is sealed or its tip is too small.	Replace the micropipette with a newly-made one.
		Use the micropipette with an appropriate tip size.
	Air bubbles are trapped in the micropipette tip.	Gently flick the micropipette to discharge bubbles or reload the injection solution.
	Large dextrans are filtered during diffusion.	Use small dextrans. 3 kDa-dextran is recommended.
	Embryos are not healthy enough.	Use another healthy embryo.
		Operate embryos as quickly as possible.
High background in the extracellular space	The micropipette tip is too large.	Adjust the tip size of micropipettes.
	The output pressure is too high or pulse duration is too long.	Reduce the output pressure or the pulse duration.