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. 2018 Mar 8;15(5):7215–7220. doi: 10.3892/ol.2018.8217

Figure 2.

Figure 2.

miR-532-5p downregulates RUNX3 expression in HT-29 CRC cells. HT-29 cells were transfected with 20 or 40 nM miR-532-5p mimic or Ctrl miRNA for 12 h. The relative miR-532-5p level to 5S rRNA (A) and the relative mRNA level of RUNX3 to GAPDH (B) were examined. (C) Western blot analysis of the RUNX3 protein level in the miR-532-5p mimic- or the Ctrl miRNA-transfected (for 24 h) HT-29 cells. (D) Expression levels of RUNX3 relative to GAPDH expression levels in HT-29 cells transfected with 20 or 40 nM miR-532-5p mimic and Ctrl miRNA. Results are the mean ± standard error of the mean from three independent experiments. *P<0.05; **P<0.01; ****P<0.0001. miR, microRNA; RUNX3, runt-related transcription factor 3; CRC, colorectal cancer; Ctrl, control.