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. 2018 Mar 8;15(5):7215–7220. doi: 10.3892/ol.2018.8217

Figure 3.

Figure 3.

Targeting inhibition by miR-532-5p on the expression of RUNX3, with the luciferase reporting assay in HT-29 cells. (A) Alignment of Homo sapiens miR-532-5p with the target sequences within the 5′UTR of H. sapiens RUNX3 and a schematic diagram of the luciferase reporter with the 5′UTR of RUNX3 (wild-type or mutant 5′UTR of RUNX3). Relative luciferase activity of the reporter with the (B) 5′UTR of RUNX3 or (C) mutant 5′UTR of RUNX3 in the HT-29 cells, following transfection with miR-532-5p mimic or with Ctrl miRNA. Experiments were performed in triplicate. **P<0.01, ns, not significant. SV40, simian virus 40; GLuc, Gaussia luciferase; SeAP, secreted embryonic alkaline phosphatase; CMV, cytomegalovirus; miR, microRNA; RUNX3, runt-related transcription factor 3; Ctrl, control; UTR, untranslated region.