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. 2018 Mar 23;15(5):8172–8180. doi: 10.3892/ol.2018.8330

Figure 4.

Figure 4.

CCNT2 is a direct target of miR-142-3p in gastric cancer cells. (A) Prediction of binding site of miR-142-3p in CCNT2 by Pictar. (B) Relative luciferase activity of 293T cells that were co-transfected with reporter constructs and miR-142-3p mimics or scrambled control. Data are expressed as the mean ± standard deviation (n=3). (C) Relative luciferase activity of 293T cells that were transfected with wild-type and mutated CCNT2 3′UTR constructs. Data are expressed as the mean ± standard deviation (n=3). **P<0.01 vs. scrambled control. (D) Western blot analysis of CCNT2 protein levels in HGC-27 and MGC-803 cells that were transfected with scrambled control or miR-142-3p mimics. CCNT2, cyclin T2; LUC, luciferase; miRNA, microRNA; mut, mutated; PC, positive control; Scr, scrambled control; UTR, untranslated region; WT, wild-type; 142-3p, miR-142-3p mimic.