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. 2018 Mar 23;15(5):8172–8180. doi: 10.3892/ol.2018.8330

Table I.

Sequence of primers used in reverse transcription-quantitative polymerase chain reaction and constructs.

Primer Sequence (5′-3′)
miR-142-3p
  RT GTCGTATCCAGTGCAGGGTCCGAGGTA
TTCGCACTGGATACGACTCCATAA
  Forward CTGTGTAGTGTTTCCTACTTTA
  Reverse GTGCAGGGTCCGAGGT
  Probe FAM-GTAGTGTTTCCTACTTTATGG-MGB
U6
  RT AAAATATGGAACGCTTCACGAATTTG
  Forward CTCGCTTCGGCAGCACATATACT
  Reverse ACGCTTCACGAATTTGCGTGTC
  Probe FAM-CCATGCTAATCTTCTCTGTA-MGB
CCNT2-UTR
  Forward ACCAGATTGGCTGCTGAA
  Reverse CCCTACAAAGAGGCTCCTAAGT

CCNT2, cyclin T2; miRNA, microRNA; RT, reverse transcription; UTR, untranslated region.