Table I.
Primer | Sequence (5′-3′) |
---|---|
miR-142-3p | |
RT | GTCGTATCCAGTGCAGGGTCCGAGGTA |
TTCGCACTGGATACGACTCCATAA | |
Forward | CTGTGTAGTGTTTCCTACTTTA |
Reverse | GTGCAGGGTCCGAGGT |
Probe | FAM-GTAGTGTTTCCTACTTTATGG-MGB |
U6 | |
RT | AAAATATGGAACGCTTCACGAATTTG |
Forward | CTCGCTTCGGCAGCACATATACT |
Reverse | ACGCTTCACGAATTTGCGTGTC |
Probe | FAM-CCATGCTAATCTTCTCTGTA-MGB |
CCNT2-UTR | |
Forward | ACCAGATTGGCTGCTGAA |
Reverse | CCCTACAAAGAGGCTCCTAAGT |
CCNT2, cyclin T2; miRNA, microRNA; RT, reverse transcription; UTR, untranslated region.