Figure 2.

HOTAIR inhibition suppressed the proliferation of HepG2 cells. HepG2 cells were infected with HOTAIR siRNA lentiviral vectors or the empty lentiviral vectors for 72 h. (A) The expression of HOTAIR was evaluated by qRT-RCR. (B) The proliferation rate was measured by CCK-8 assay. (C and D) The cell number was assessed by optical microscope. (E) The protein expression of two proliferation marker proteins Ki67 and PCNA was evaluated by western blotting. GAPDH was used as an internal control (*P<0.05, ***P<0.001). HOTAIR, HOX transcript antisense RNA.