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. 2017 Jun 20;98(6):1282–1293. doi: 10.1099/jgv.0.000815

Fig. 5.

Fig. 5.

DI-free SeV is a weak activator of ISRE reporter. (a) Immunostimulatory activity of HA-RIG-I associated RNA from 293 T cells infected with either SeVΔC or SeV-GFP. In the right panel, a Western blot shows the expression levels of HA-RIG-I in both infections. (b) 293T ISRE-RFP reporter cells were infected with either SeVΔC or SeV-GFP and infections were allowed to proceed for 24 h. Red fluorescent protein (RFP) expression correlates to the immunostimulatory potential of each virus. (c) Infection of ISRE-RFP cells with SeV-GFP results in minimal RFP expression isolated to a few cells over the first 72 h of infection. (d) Individual RFP plaques surrounding SeV-GFP-infected cells at 96h post-infection. The bottom panel is a magnified view of the images in the top panel. The single yellow cell in the middle of the merged image represents a double-positive cell.