Fig. 6.

Knockdown of SeV C protein in infected cells. HeLa cells were transfected with siGFP or siCNTRL and infected with SeV-GFP-C (which expresses a GFP-C fusion protein). (a) Sendai RNA-Seq reads from siRNA-transfected and SeV- or mock-infected cells. The top two panels highlight very similar viral profiles in the presence or absence of C protein. (b) Comparison of loss of GFP RNA versus GFP protein, illustrating that the majority of the GFP protein is removed in siGFP cells. (c) RNA-Seq reads from siRNA-treated and infected cells were mapped to the human IFNB gene. Very strong IFNB expression can be seen in infected cells with GFP-C knockdown.