Table 1. Description of cbDIs associated with various virus stocks and identified through RNA-Seq analysis.
RNA-Seq fusion reads were used to deduce the exact nature of the cbDIs associated with each listed virus. The exact fusion point of the cbDI, its overall length, and the length of the dsRNA panhandle are listed. The percentage of reads supporting fusion is reflective of the overall prevalence of a particular cbDI within the total viral RNA pool.
| Virus | Fusion point | DI length (nt) | dsRNA length (nt) | No. of fusion reads | No. of total viral reads | Percentage of reads supporting fusion point |
|---|---|---|---|---|---|---|
| SeV-Cantell | 14931/15291 | 546 | 92 | 121 248 | 23 510 595 | 0.5 |
| 14984/15304 | 480 | 80 | 148 | 0.0006 | ||
| 14950/15278 | 540 | 106 | 336 | 0.001 | ||
| SeV-ΔC | 14258/15280 (DI1) | 1230 | 104 | 8435 | 16 361 704 | 0.05 |
| 14931/15291 | 546 | 92 | 49 | 0.0003 | ||
| 14984/15304 (DI2) | 480 | 80 | 127 405 | 0.8 | ||
| 15178/15284 | 306 | 100 | 869 | 0.005 | ||
| SeV-GFP-C | 14931/15291 | 546 | 92 | 38 | 16 040 655 | 0.0003 |
| 14984/15304 | 480 | 80 | 59 | 0.0004 | ||
| SeV-GFP | 14931/15291 | 546 | 92 | 24 | 5 770 180 | 0.0004 |
| 14984/15304 | 480 | 80 | 19 | 0.0003 | ||
| SeV-GFP-P6 | 14931/15291 | 546 | 92 | 49 | 17 478 494 | 0.0002 |
| 14984/15304 | 480 | 80 | 33 | 0.0002 |