Fig. 3.

Repression of PRRSV infection by mTOR inhibitors in porcine alveolar macrophages and mDCs. Cells were incubated with DsRed-labelled PRRSV (m.o.i. of 0.5) for 1 h, and then treated with mTOR inhibitors (rapamycin or PP242) or activator (MHY1485) at indicated concentrations for another 20 h. Infected macrophages (a) and mDCs (b) were quantified using a SpectraMax i3 at 20 h p.i. n=4; a, b and c indicate p<0.001, 0.01 and 0.05, respectively, relative to the control. Rapa, Rapamycin; MHY, MHY1485.