Fig. 4.

mTOR inhibitors or mTOR activator modulate gene expression of type I IFNs and their receptors in PRRSV-infected porcine alveolar macrophages. Cells were incubated with DsRed-labelled PRRSV (m.o.i. of 0.5) for 1 h, and then treated with mTOR inhibitors (rapamycin 1 µM or PP242 2 µM) or activator (MHY1485 2 µM) for 12 h. Total RNAs were extracted to analyse expression of type I IFNs and their receptors in cells infected with PRRSV or not (a) and PRRSV-infected cells with/without inhibitor or activator treatments (b) by specific primers using two-step RT-PCR. Control was normalized to 0; n=3; a, b and c indicate p<0.001, 0.01 and 0.05, respectively to the control.