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. 2017 Jun 14;98(6):1316–1328. doi: 10.1099/jgv.0.000802

Fig. 6.

Fig. 6.

Suppression of Rictor or Raptor gene expression differently suppresses PRRSV replication in MARC-145 cells. Gene expression of Rictor and Raptor was down-regulated using the CRISPR/Cas9 system. (a,b) Gene expression analysis to show Rictor or Raptor gene expression was down-regulated nearly 60 % dependent on the transformation efficiency (>90 % of gRNA). (c) MARC-145-sgControl/sgRictor/sgRaptor cells were infected with DsRed-labelled PRRSV (m.o.i. of 1), visualized and imaged with fluorescence microscopy at 36 h p.i. n=3; a, b and c indicate p<0.001, 0.01 and 0.05, respectively, relative to the control.