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. 2017 Jun 14;98(6):1316–1328. doi: 10.1099/jgv.0.000802

Fig. 7.

Fig. 7.

Suppression of Rictor, the key subunit of mTORC2, significantly suppresses PRRSV replication in MARC-145 cells, through regulating type I IFN production and action signalling. MARC-145-sgControl/sgRictor/sgRaptor cells were infected with DsRed-labelled PRRSV (m.o.i. of 1), and quantified using a SpectraMax i3 at 36 h p.i. (a); or infected with DsRed-labelled PRRSV for 24 h. Then cell culture supernatants were collected and PRRSV was inactivated with UV illumination. (b–d) Supernatants were used to detect IFN production and action with a bioassay in MARC-145 cells stably transformed with an IRF3-, IRF7- or Mx1-promoter driven luciferase reporter system; n=3; a, b and c indicate p<0.001, 0.01 and 0.05, respectively, relative to the control.