FIG. 2.
Effect of MCC950 on expression of principal constituents of NLRP3 inflammasome at 24 and 72 h post-TBI. (A) Representative and quantitative analysis of (B) NLRP3, (C) cleaved caspase-1, (D) ASC, and (E) IL-1β expression demonstrate the corresponding differences in pericontusional cerebral cortex at 24 and 72 h post-TBI. MCC950 treatment post-injury reduced levels of NLRP3 and subsequent caspase-1 and IL-1β maturation. Values are expressed as mean ± SEM (n = 8). *p < 0.05 versus corresponding sham; **p < 0.01 versus corresponding sham; #p < 0.05 versus corresponding TBI; ##p < 0.01 versus corresponding TBI. ASC, apoptosis-associated speck-like; IL-1β, interleukin-1 beta; NLRP3, nucleotide oligomerization domain (NOD)-like receptor protein-3; SEM, standard error of the mean; TBI, traumatic brain injury; C1, caspase-1, cleaved caspase-1.