Figure 5.

Postinfection administration of MAb to PNAG reduces bacterial burdens and conjunctival pathology due to S. pneumoniae (D-39 and ATCC8) or S. aureus (LAC) in WT C57Bl/6, but not in RAG 1 KO or germ-free mice (GFM) after 48 hours. A total of 10 μg of control IgG MAb (□) or MAb to PNAG (▵) were injected into the conjunctiva 4, 24, and 32 hours postinfection. Mice (n = 5 per group) were euthanized at 48 hours postinfection and pathology scores (upper row) and CFU/conjunctiva (lower row) were determined. Symbols represent individual animals, bars the median, and error bars the 95% CI, and one-sided P values were determined by nonparametric t-tests. None of the differences between MAb to PNAG or control MAb in RAG 1 KO or GFM mice were significant (P > 0.22).