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. 2018 May 15;9:936. doi: 10.3389/fmicb.2018.00936

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Copyright © 2018 Wang, Li, Liu, Chang, Sun, Gao, Wang, Zhou, Liu, Zheng, Cui, Chen, Li, Yuan, Wen, Peng and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blotting and silver staining of exogenously expressed PA-N155 and PA-N182 in chicken cells. (A) FLAG-tagged PA-N155 and PA-N182 were transfected into chicken DF1 cells and expression was determined 48 h after transfection. β-Actin was used as a loading control. (B) Eluates of triplicate FLAG immunoprecipitates from chicken DF1 cells were subjected to SDS-PAGE and silver stained. FLAG-tagged PA-N155 and PA-N182 proteins are indicated by the red arrows.