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. 2018 May 21;9:144. doi: 10.1186/s13287-018-0885-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Analysis of clone formation, membrane potential, and cell cycle of MM CSCs. As described in the Methods, 1 × 10⁶ MM CSCs treated with various agents for 30 min were used for assay clone formation, membrane potential, and cell cycle analysis. a Images showing clone formation rate. c,e Changes in mitochondrial membrane potential and cell cycle were analyzed by FCM. b,d,f Statistical analysis of the clone formation rate and changes in mitochondrial membrane potential and cell cycle. *P < 0.05, **P < 0.01, ***P < 0.005. EPI, epirubicin; mAb, monoclonal antibody; MB, microbubble; US, ultrasound