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. 2018 May 21;9:144. doi: 10.1186/s13287-018-0885-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Expression of apoptosis-related molecules in tumor tissues analyzed with immunohistochemistry and Western blot. a,e Images of apoptosis-related molecule expression, including Caspase-3, Bax, TUNEL, PCNA, Bcl-2, and CD31 analyzed with immunohistochemistry (a, original magnification ×400; e, original magnification ×100). b–d,f–h Quantification analysis of Caspase-3, Bax, TUNEL, PCNA, Bcl-2, and CD31 expression. i Expression of P-p65and P-IκBα analyzed by Western blot. j Semiquantification analysis of molecular expression. *P < 0.05, **P < 0.01. EPI, epirubicin; mAb, monoclonal antibody; MB, microbubble; US, ultrasound