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. 2018 May 21;18:578. doi: 10.1186/s12885-018-4463-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Sanguinarine induced apoptosis of CRC cells by inhibition of the interaction of MELK and STRAP. a SW480 cells were treated with sanguinarine for 12 h. STRAP and MELK mRNA relative level was detected by qRT-PCR. b Western blotting analysis of MELK, STRAP, p-MELK and p-STRAP in SW480 cells after treatment of 2.4 μM sanguinarine for specified times. c The in vitro kinase activity of MELK was analyzed by using HTRF^® KinEASE™-STK kit. d The interaction between MELK and STRAP was analyzed by Co-IP. Cells were lysed and immunoprecipitated using an anti-MELK or STRAP antibody. The precipitated proteins were analyzed using westernblotting. Data presented are showed as means ± SD from three independent experiments. ^# p < 0.01 indicates significant difference