Figure 5.

Effect of leptin treatment on Ob-Rb/STAT pathway and autophagy in avian muscle (QM7) cells. QM7 cells were treated with recombinant ovine leptin (100 ng/mL) for 24 h and protein levels of Ob-Rb, STAT3, and autophagy pathways were measured by Western blot (A,D). Their relative expression was presented as normalized ratio to pan STAT3 or actin (B,E). Their mRNA abundances were measured by qPCR (C,G). Autophagosome and autolysosome formation as well as autophagy-related protein abundance were determined also by immunofluorescence (F). Data are presented as mean ± SEM (representative of 3 experiments, n = 6). ^*Indicates significant difference at P < 0.05.