Table 1.

Oligonucleotide real-time qPCR primers.

Gene	Accession numbera	Primer sequence (5′ → 3′)	Orientation	Product size (bp)
Atg3	NM_001278070	GAACGTCATCAACACGGTGAA TGAGGACGGGAGTGAGGTACTC	Forward Reverse	65
Atg7	NM_001030592	CGATGAACCCAAAAGGTCAGA ACTGGCAATGCGTGTTTCAG	Forward Reverse	57
Beclin 1	NM_001006332	TGCATGCCCTTGCTAACAAA CCATACGGTACAAGACGGTATCTTT	Forward Reverse	61
LC3a	XM_417327	CCTTGTCCCAGACCATGTCAA AGCGGCGCCGGATT	Forward Reverse	56
LC3b	NM_001031461	AACTCCAACCAGGCCTTCTTC GTGGAGACGCTCACCATGCT	Forward Reverse	59
LC3c	XM_419549	CCTGTTGGACAAAACCAAGTTTC ATGGTGATGAACTGCGTCATG	Forward Reverse	63
Ob-Rb	NM_204323	GCAAGACCCTCTCCCTTATCTCT TCTGTGAAAGCATCATCCTGATCT	Forward Reverse	70
STAT1	NM_001012914	AGTTCACCAGCTGTACGATGACA TCCAGCCACTGTGCCAAGTA	Forward Reverse	66
STAT3	AY641397	GCATGTCGTTTGCGGAAAT CAGAATGTTGGTGGCATCCA	Forward Reverse	59
STAT5	AF074248	TCACCATCGCGTGGAAGTT GGTGGTGAAAGGCATCAGGTT	Forward Reverse	65
STAT6	XM_003643566	AGGCAGCTGCGCAACCT ATCCGAACGCCTGATCCTT	Forward Reverse	52

^aAccession number refer to Genbank (NCBI).

Atg3 and 7, autophagy related gene; LC3a, b, and c, microtubule associated protein 1 light chain 3; Ob-Rb, leptin receptor; STAT, signal transducer and activator of transcription.