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. 2018 May 21;15:89. doi: 10.1186/s12985-018-1002-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The doses of pAAV/HBV1.2 plasmid determine HBV persistence in C57BL/6 mice. C57BL/6 mice were hydrodynamically injected with 6 μg or 20 μg pAAV/HBV1.2 plasmid. Serum levels of HBsAg (a) and HBeAg (b) were measured at indicated time points. c Positive rate of serum HBsAg after HBV plasmid injection. d Immunohistochemical staining for HBcAg in liver sections of mice at week 6 after injection with 6 μg or 20 μg pAAV/HBV1.2 plasmid. (original magnification: 100×). Results represent 2 independent experiments (n = 10/group)