Fig. 2.

Confirmation of the block of macroautophagy by treatment with BAF in oligodendroglial cells. Cell lysates were analyzed by Western blotting and the band intensities for LC3B-II were normalized to LC3B-I levels. Increased LC3B-II levels indicate the successful block of the fusion of the autophagosome with the lysosome. Oligodendroglial cells untreated or treated with 18 µg/mL sol or fib α-syn for 24 h showed a low ratio of LC3B-II/LC3B-I, whereas BAF-treated oligodendroglia revealed significantly higher levels of the LC3B-II/LC3B-I ratio independent of the α-syn treatment as compared to oligodendroglia not treated with BAF. Two-way analysis of variance with post hoc Bonferroni test was applied. Data are presented as mean ± SEM. ***p < 0.001. N number equals 4