Fig. 3.

Blocking macroautophagy with BAF in combination with α-syn treatment results in increased ROS production in oligodendroglial cells. Following treatment, cells were stained for ROS by using nitroblue tetrazolium chloride (NBT) resulting in a blue precipitate in the presence of ROS. We found that treatment with BAF significantly enhances the number of NBT-positive oligodendroglial cells compared to untreated cells. Furthermore, the additional treatment with extracellular fib α-syn for 24 h revealed a significant increase of NBT-positive cells compared to cells treated with BAF only and cells not treated with BAF. However, treatment with sol α-syn did not induce a significant increase of ROS production. Arrows pointing out oligodendroglial cells positively stained for NBT. Two-way analysis of variance with post hoc Bonferroni test was applied. Data are presented as mean ± SEM. **p < 0.01; ***p < 0.001. Scale bar 20 µm. N number equals 4