Fig. 5.

Incorporation of extracellular monomeric sol and fib α-syn species and inclusion formation in oligodendroglial cells upon macroautophagy blocking with BAF. Intracellular amounts of uptaken α-syn monomers and endogenous α-syn were measured in oligodendroglial cells challenged with extracellular sol or fib α-syn for 24 h combined with macroautophagy blocking for 24 h using Western blot analysis. α-syn levels were normalized to actin. Addition of sol and fib α-syn led to a significantly increased amount of α-syn in oligodendroglial cells (24 kDa band due to his-tag). Moreover, an increased amount of α-syn was found in oligodendroglia treated with BAF compared to cells not treated with α-syn, however a significant change was detected only after fib α-syn exposure. Comparing untreated cells/cells treated with BAF and α-syn a not significant decrease regarding the amounts of α-syn was measured (a, c). Levels of endogenously expressed α-syn (14 kDa band, no his-tag) remained the same regarding all relevant treatments (b, c). Two-way analysis of variance with post hoc Bonferroni test was applied. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01. N number equals 4