Fig. 2.

Gene expression of GM-CSF, N-α-Syn, or GM-CSF/N-α-Syn-cultured BMDCs. GM-CSF-generated BMDCs were cultured for 2 days in media or 20 ng/ml GM-CSF (GM-CSF). BMDC cultures from each group were stimulated with 30 μg/ml N-α-Syn for 6 h (N-α-Syn or GM-CSF + N-α-Syn, respectively). RNA was isolated, converted to cDNA and assessed by PCR arrays for proinflammatory genes. Gene expression from BMDCs from treatment groups was determined relative to BMDCs treated with media alone. Each treatment group was composed of 6 replicates and fold change was determined by SA Bioscience array software