Table 2.
F. novicida mutants: Protection against F. tularensis subsp. tularensis challenge.
| Vaccine | Host straina | Vaccine route, dose (CFU)b | Boost (route) | LVS control (route) | Interval (days)c | SCHU S4 challenge route, dose (CFU or LD50)d | % Survival post-challenge (MST, days)e | References | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Vaccine | LVS | Sham | ||||||||
| U112 | BALB/c | i.d., 100 | No | Yes (i.d.) | 56 | Aerosol, 10§ | 0 (5) | 0 (6) | 0 (5) | Shen et al., 2004 |
| U112 | BALB/c | i.d., 100 | No | Yes (i.d.) | 56 | i.d., 350§ | 0 (5) | 100 (>21) | 0 (6) | |
| U112 | Rats | i.t., 107 | No | No | 30 | i.t., 25 LD50 | 100 | ND | 0 (10) | Signarovitz et al., 2012 |
| p.o., 107 | No | No | 30 | i.t., 25 LD50 | 50 | ND | 0 (10) | Signarovitz et al., 2012 | ||
| ΔiglB (KKF235) |
C57BL/6 | p.o., 103 | No | No | 21 | i.n., 25 | 67 | ND | 0 (6) | Cong et al., 2009 |
| C57BL/6 | p.o., 103 | No | No | 21 | i.n., 50 | 10 | ND | Cong et al., 2009 | ||
| C57BL/6 | p.o., 103 | Yes (p.o.) | No | 21 | i.n., 52 | 40 | ND | Cong et al., 2009 | ||
| ΔiglB | Rats | i.t., 107 | No | No | 30 | i.t., 1.25 × 104* | 50 | ND | 0 (10) | Signarovitz et al., 2012 |
| p.o., 107 | No | No | 30 | i.t., 1.25 × 104* | 50 | ND | 0 (10) | Signarovitz et al., 2012 | ||
| ΔiglB::fljB | BALB/c | p.o., 107 | No | No | 30 | i.t., 1.25 × 104* | 83 | ND | Cunningham et al., 2014 | |
| Rats | p.o., 107 | No | No | 30 | i.t., 1.25 × 104* | 88 | ND | Cunningham et al., 2014 | ||
| Fn iglD | BALB/c | i.n., ~109 | No | No | 30 | i.n., 103 | 0 | ND | 0 | Chu et al., 2014 |
| Rats | p.o., 107 | No | No | 30 | i.t., 104 | 83 | ND | 17 | Chu et al., 2014 | |
| Rats | i.t., 105 | No | No | 30 | i.t., 104 | 100 | ND | 25 | Chu et al., 2014 | |
| Rats | i.t., 107 | No | No | 30 | i.t., 104 | 83 | ND | 25 | Chu et al., 2014 | |
| NHP | t.b., 108 | No | Yes (s.c.) | 30 | Aerosol, (2,500–5,000) | 83 | 100 | 0 (8) | Chu et al., 2014 | |
| ΔpmrA | BALB/c | i.n. (106) | No | No | 35 | i.n., 100 | Not protected | ND | ND | Mohapatra et al., 2007 |
a
Host strain: Mice: BALB/c or C57BL/6 mice; Rats: Fisher rats; NHP: non-human primates, cynomolgus macaques.
b
Vaccine route: intranasal (i.n.); oral (p.o.); intratracheal (i.t.); via bronchoscopy (t.b.).
c
Interval: interval between the only or the last vaccination and challenge.
d
SCHU S4 challenge route (dose, CFU of LD50):
§
challenged with subsp. tularensis FSC 033 strain; *,1.25 × 104 CFU, approximately 25 LD50 in rats.
e
% Survival post-challenge: % survival post-challenge in animals immunized with the vaccine candidate (vaccine), LVS control (LVS), or PBS or unvaccinated control (Sham). MST, mean/median survival time. ND; not determined.