FIGURE 6.

Influence of IHF on tssD2a-lux and tssD2b-lux expression. Overnight cultures of the V. fluvialis strains 85003 (WT), ΔihfA or ΔihfB each containing either ptssD2a-lux or ptssD2b-lux reporter fusions were diluted 1:100 in LB medium and 200 μl aliquots were transferred to Opaque-wall 96-well microtiter plates. The plates were incubated at 30°C with shaking for measuring the OD₆₀₀ and light units. Luminescence activity is calculated as light units/OD₆₀₀. The data represent three independent experiments. ^∗∗Significantly different from the WT (t-test, P < 0.05).