Table 1.
Strains and plasmids used in this study.
| Strain/plasmid | Characteristics | Reference/source |
|---|---|---|
| E. coli | ||
| DH5αλpir | sup E44ΔlacU169 (ΦlacZΔM15) recA1 endA1 hsdR17 thi-1 gyrA96 relA1pir (Laboratory stock) | Mekalanos Laboratory (Harvard Medical School) |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2Tc::Mu (λpirR6K), KmR | Mekalanos Laboratory (Harvard Medical School) |
| MG1655 | K-12 F- λ- ilvG- rfb-50 rph-1, RfpR | Laboratory stock |
| V. fluvialis | ||
| 85003 | V. fluvialis, wild-type, SmR | Lu et al., 2014 |
| ΔihfA | 85003, ΔihfA | This study |
| ΔihfB | 85003, ΔihfB | This study |
| ΔvasH | 85003, ΔvasH | Huang et al., 2017 |
| ΔihfA/pSRihfA | ΔihfA containing complemented plasmid pSRihfA | This study |
| ΔihfA/pSRKTc | ΔihfA containing control vector pSRKTc | This study |
| ΔihfB/pSRihfB | ΔihfB containing complemented plasmid pSRihfB | This study |
| ΔihfB/pSRKTc | ΔihfB containing control vector pSRKTc | This study |
| Plasmid | ||
| pWM91 | Suicide vector containing R6K ori, sacB, lacZα; AmpR | Laboratory stock |
| pSRKTc | Broad-host-range vector containing lac promoter, lacIq, lacZα,TetR | Khan et al., 2008 |
| pBBRlux | bioluminescence based reporter plasmid containing a promoterless luxCDABE operon; CmR | Wu et al., 2015 |
| pWM-ΔihfA | 1.69 kb BamHI-XhoI ΔihfA fragment of V. fluvialis in pWM91 | This study |
| pWM-ΔihfB | 1.70 kb BamHI-XhoI ΔihfB fragment of V. fluvialis in pWM91 | This study |
| pSRihfA | 313 bp NdeI-XhoI ihfA ORF of V. fluvialis in pSRKTc | This study |
| pSRihfB | 304 bp NdeI-XhoI ihfB ORF of V. fluvialis in pSRKTc | This study |
| ptssD2a-lux | 375 bp SacI-BamHI fragment of tssD2_a promoter region in pBBRlux | This study |
| ptssD2aM-lux | 375 bp SacI-BamHI fragment of tssD2_a promoter with IHF consensus mutation in pBBRlux | This study |
| ptssD2b-lux | 375 bp SacI-SpeI fragment of tssD2_b promoter region in pBBRlux | This study |
| ptssD2c-lux | 604 bp SacI-BamHI fragment of tssD2_c promoter region in pBBRlux | This study |
| ptssD2c′-lux | 395 bp SacI-BamHI fragment of shortened tssD2_c promoter region in pBBRlux | This study |
| pVflT6SS2-lux | 450 bp SacI-BamHI fragment of VflT6SS2 major cluster promoter region in pBBRlux | This study |
| pVflT6SS2-lux-ihf1M | 450 bp SacI-BamHI fragment of VflT6SS2 promoter with mutations in the first IHF binding site in pBBRlux | This study |
| pVflT6SS2-lux-ihf2M | 450 bp SacI-BamHI fragment of VflT6SS2 promoter with mutations in the second IHF binding site in pBBRlux | This study |
| pVflT6SS2-lux-ihf3M | 450 bp SacI-BamHI fragment of VflT6SS2 promoter with mutations in the third IHF binding site in pBBRlux | This study |
| pVflT6SS2-lux-ihf1+2M | 450 bp SacI-BamHI fragment of VflT6SS2 promoter with mutations in the first and second IHF binding sites in pBBRlux | This study |